The Estimation of Galactose in Plasma Using Galactose Oxidase
نویسندگان
چکیده
منابع مشابه
The Estimation of Galactose in Plasma Using Galactose Oxidase.
The estimation of galactose in plasma by the enzyme system galactose oxidaseperoxidase was investigated. The method was found to be relatively specific for galactose although xylose and ascorbic acid reacted to a slight degree with the enzyme; lactose also reacted with the enzyme, probably because partial hydrolysis of the disaccharide released galactose. The relationship between absorbance and...
متن کاملCofactor processing in galactose oxidase.
GO (galactose oxidase; E.C. 1.1.3.9) is a monomeric 68 kDa enzyme that contains a single copper ion and an amino acid-derived cofactor. The enzyme is produced by the filamentous fungus Fusarium graminearum as an extracellular enzyme. The enzyme has been extensively studied by structural, spectroscopic, kinetic and mutational approaches that have provided insight into the catalytic mechanism of ...
متن کاملGalactose oxidase action on galactose containing glycolipids--a fluorescence method.
Features that alter the glycolipid sugar headgroup accessibility at the membrane interface have been studied in bilayer lipid model vesicles using a fluorescence technique with the enzyme galactose oxidase. The effects on oxidation caused by variation in the hydrophobic moiety of galactosylceramide or the membrane environment for galactosylceramide, monogalactosyldiacylglycerol and digalactosyl...
متن کاملThe D-galactose oxidase of Polyporus circinatus.
The mold Polyporus circinatus produces an extracellular oxidase that catalyzes the oxidation of n-galactose (1). The reaction appears superficially to be similar to the oxidation of n-glucose catalyzed by notatin (2)) and can be followed by measuring the consumption of oxygen or the production of Hz02 (1). The enzyme is useful for the determination of galactose, employing the same peroxidase-ch...
متن کاملThe radical chemistry of galactose oxidase.
Galactose oxidase is a free radical metalloenzyme containing a novel metalloradical complex, comprised of a protein radical coordinated to a copper ion in the active site. The unusually stable protein radical is formed from the redox-active side chain of a cross-linked tyrosine residue (Tyr-Cys). Biochemical studies on galactose oxidase have revealed a new class of oxidation mechanisms based on...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1964
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/10.11.1002